Representative ICEs
ICEs 1, 2 are primarily resided in the bacterial cell's chromosome and have the ability to be transferred between cells by conjugation, which function is self-encoded. The core components of an ICE typically include attL (attachment site at the left end of the ICE), int [integrase: tyrosine or serine recombinase, or a DD(35)E transposase], xis (excisionase), rlx/mob (TraI-family relaxase), nic/oriT (nick site, origin of conjugative replication), cpl (TcpA- or VirD4-family coupling protein), a P (TivB)- or F (TivF)-type T4SS machinery (mating pair formation), and attR (attachment site at the right end of the ICE). A complete gene set for conjugal transfer include rlx/mob, nic/oriT, cpl, and the P- or F-type T4SS genes. ICEs are autonomous in both integration and conjugation.
Table 1|List of representative ICEs
| Category | Sub-category | Designation | Accession number | Synonym | Resistance genes | DR | FASTA sequence | Gene list | GBK-annotation | SQN-annotation | Manual annotation | Gene organization |
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| Integrative and conjugative element (ICE) | Tn916 | U09422 | tet(M) |  |
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| Integrative and conjugative element (ICE) | Tn6397 | CP021851 | aacA4'-3, and blaIMP-8 | |
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| Integrative and conjugative element (ICE) | Tn6398a | KC543497 | ble | |
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| Integrative and conjugative element (ICE) | Tn6417 | CP013993 | aadB | |
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| Integrative and conjugative element (ICE) | Tn6430 | CP009975 | None | |
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| Integrative and conjugative element (ICE) | Tn6484 | KY494864 | acrAB–tolC | |
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| Integrative and conjugative element (ICE) | Tn6511 | AL009126 | ICEBs1 | aacA4'-3, and blaIMP-8 | |
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| Integrative and conjugative element (ICE) | Tn6512 | AY090559 | R391 | aphA1 | |
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| Integrative and conjugative element (ICE) | Tn6521 | AJ617740 | ICEclc | None | |
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| Integrative and conjugative element (ICE) | Tn6575 | MH160822 | aacA4cr, aacC4, ampR, ampC, aphA1, aph(4)-Ia, arr-3, blaOXA-1, blaNDM-1, bleMBL, bleO, catB3, cfr, qacED1, sul1, tetA(C), and tetR(C) | |
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| Integrative and conjugative element (ICE) | Tn6576 | CP042858 | aadA2, ereA, dfrA32, chrA, bleO, aacA4cr, blaOXA-1, catB3, arr-3, qacED1, sul1, aacC4, aph(4)-Ia, sul2, floR, blaCTX-M-14, aphA1, tetR(C), tetA(C), strA, strB, mph(A), aacC2, tmrB, sil, cop, merD, merE, blaTEM-1, blaNDM-3, and bleMBL | |
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| Integrative and conjugative element (ICE) | Tn6577 | CP042857 | sul2, blaHMS-1, tetA(C), tetR(C), blaCTX-M-14, floR, aph(4)-Ia, aacC4, aacA4cr, blaOXA-1, catB3, arr-3, qacED1, sul1, bleO, chrA, dfrA32, ereA, and aadA2 | |
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| Integrative and conjugative element (ICE) | Tn6582 | CP042858 | blaNDM-1, blaOXA-1, strA, bleMBL, strB, sul2, floR, strA, aphA1, aacC2, bleO, chrA, dfrA27, sul1, aadA2, aacA4-cr, aadA16, arr-3, sil, cop, tmrB, qacED1, chrA, mer, blaTEM-1, catB3, and mph(A) | |
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| Integrative and conjugative element (ICE) | Tn6786 | CP046404 | crpP-1.1 | |
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| Integrative and conjugative element (ICE) | Tn6828 | CP011317 | crpP-1.2 | |
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| Integrative and conjugative element (ICE) | Tn6967 | CP064827 | macB, floR, sul2, aph(4)-Ia, aacC4, aacA4cr, blaOXA-1, catB3, arr-3, qacED1, and sul1 | |
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| Integrative and conjugative element (ICE) | Tn6879 | CP033104 | |
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| Integrative and conjugative element (ICE) | Tn7458 | CP081345 | blaGES-5, aacA4', aphA15, qacED1, cmlA9, tetA(G), and mer | |
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| Integrative and conjugative element (ICE) | Tn7459 | CP096941 | blaGES-5, aacA4', aphA15, qacED1, cmlA9, tetA(G), and mer | |
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| Integrative and conjugative element (ICE) | Tn7461 | CP096950 | adfrA16, aadA2, qacED1, sul1, erm42, strB, strA, and mer | |
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| Integrative and conjugative element (ICE) | Tn7462 | CP096946 | blaOXA-10, aadA1e, qacED1, sul1, and mer | |
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| Integrative and conjugative element (ICE) | Tn7463 | CP096929 | blaOXA-10, aadA1e, qacED1, sul1, mer locus, qnrVC1, and aacA4' | |
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| Integrative and conjugative element (ICE) | Tn7464 | CP096937 | blaOXA-10, aadA1e, qacED1, sul1, mer locus, qnrVC1, and aacA4' | |
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| Integrative and conjugative element (ICE) | Tn7465 | CP096932 | blaOXA-10, aadA1e, qacED1, sul1, mer locus, qnrVC1, and aacA4' | |
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| Integrative and conjugative element (ICE) | Tn7466 | CP096958 | aadB, aadA2, qacED1, sul1, and mer | |
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| Integrative and conjugative element (ICE) | Tn7482 | CP096934 | blaIMP-10, and mer | |
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| Integrative and conjugative element (ICE) | Tn7486 | CP096960 | blaGES-15,aacA4', aphA15, qacED1, cmlA9, tetA(G), and mer | |
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References
1. Delavat F, Miyazaki R, Carraro N et al. The hidden life of integrative and conjugative elements. FEMS Microbiol Rev 2017; 41: 512-37.
2. Botelho J, Schulenburg H. The role of integrative and conjugative elements in antibiotic resistance evolution. Trends Microbiol 2020.